FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_DUPD1_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_DUPD1_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11506086
Sequences flagged as poor quality0
Sequence length25
%GC4

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT10775009.364609303285237No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA5334754.6364593485569285No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2375322.0644031341326667No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1907841.6581138016872115No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1133300.9849570044931003No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1013390.8807425913555661No Hit
TTTTTATTTTTTTTTTTTTTTTTTT999210.8684186786019156No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA920410.7999331831867065No Hit
TTTATTTTTTTTTTTTTTTTTTTTT830910.7221482613635949No Hit
TTATTTTTTTTTTTTTTTTTTTTTT658810.5725752440925611No Hit
TTTTATTTTTTTTTTTTTTTTTTTT593290.5156314666864128No Hit
TTTTTTATTTTTTTTTTTTTTTTTT562960.48927150379373147No Hit
TATTTTTTTTTTTTTTTTTTTTTTA549700.47774716789010613No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT543100.4720110730964466No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC532480.4627811751102851No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT499680.4342745221963403No Hit
TTTTTTTATTTTTTTTTTTTTTTTT466020.40502043874867616No Hit
TTTTTATTTTTTTTTTTTTTTTTTA460190.39995355501427676No Hit
TTTTTTTTTTTTTTTTTTTTTTATT430680.3743062584444441No Hit
TTTTTTTTATTTTTTTTTTTTTTTT399250.3469902797528195No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC381220.3313203117028675No Hit
TTTATTTTTTTTTTTTTTTTTTTTA380780.3309379053832902No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG336640.292575598687512No Hit
TTATTTTTTTTTTTTTTTTTTTTTA308860.2684318542378355No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA306710.2665632779035373No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG292660.2543523488352164No Hit
TTTTATTTTTTTTTTTTTTTTTTTA274650.23869976289069977No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA271340.2358230244411523No Hit
TATTTTTTTTTTTTTTTTTTTTTAA256780.2231688516842304No Hit
TTTTTTATTTTTTTTTTTTTTTTTA252210.219197040592257No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC250470.2176847974193831No Hit
TTTTTTTTTTTTTTTTTTTTTAATT247190.21483413212798863No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC220570.19169854979356143No Hit
TTTTTTTATTTTTTTTTTTTTTTTA216190.18789186870322366No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA210420.1828771312851303No Hit
TTTTTATTTTTTTTTTTTTTTTTAA202040.17559402910772612No Hit
TATTTTTTTTTTTTTTTTTTTTTAT191700.16660748059765937No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC191540.1664684237541767No Hit
TATATTTTTTTTTTTTTTTTTTTTT186220.16184478370837832No Hit
TATTTATTTTTTTTTTTTTTTTTTT184380.16024563000832776No Hit
TTTTTATTTTTTTTTTTTTTTTTAT183510.1594895084218908No Hit
TTTTTTTTATTTTTTTTTTTTTTTA182160.1583162163050059No Hit
TTTTTTTTTATTTTTTTTTTTTTTT182140.15829883419957055No Hit
TTTATATTTTTTTTTTTTTTTTTTT174380.15155457729066166No Hit
AATTTTTTTTTTTTTTTTTTTTTTT166260.14449744248391677No Hit
TTTATTTTTTTTTTTTTTTTTTTAA166200.1444452961676108No Hit
TTTATTTTTTTTTTTTTTTTTTTAT154690.13444189448957708No Hit
TTTTTTTTTTATTTTTTTTTTTTTT148080.12869710864319978No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT142470.12382142806858909No Hit
TTATTTTTTTTTTTTTTTTTTTTAA137840.11979747066030969No Hit
TTATTATTTTTTTTTTTTTTTTTTT133710.11620806588791358No Hit
TTTTTTTTTTTATTTTTTTTTTTTT131170.1140005384976264No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA129020.11213196216332817No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA128640.11180170216005686No Hit
TATTATTTTTTTTTTTTTTTTTTTT125420.10900318318496838No Hit
TTTTTTTTTTTTTTTTTTTTTTACC122600.10655230631858653No Hit
TTATTTTTTTTTTTTTTTTTTTTAT118280.10279777154455477No Hit
TTTTTATATTTTTTTTTTTTTTTTT117830.10240667417225979No Hit
TTTTATTTTTTTTTTTTTTTTTTAA117670.10226761732877715No Hit
TAATTTTTTTTTTTTTTTTTTTTTT116990.10167662574397585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)