FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_AKR1C4_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_AKR1C4_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10574718
Sequences flagged as poor quality0
Sequence length25
%GC4

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT8992768.5040187360079No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4626584.375133218682522No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2053841.9422172770942923No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1682151.5907279986095135No Hit
TATTTTTTTTTTTTTTTTTTTTTTT937590.8866335726399512No Hit
TTTTTATTTTTTTTTTTTTTTTTTT875650.8280599066566126No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG860810.8140264355040011No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA796680.7533817923087878No Hit
TTTATTTTTTTTTTTTTTTTTTTTT722950.6836588928423434No Hit
TTATTTTTTTTTTTTTTTTTTTTTT572890.5417543995026628No Hit
TTTTATTTTTTTTTTTTTTTTTTTT528940.5001930075109332No Hit
TTTTTTATTTTTTTTTTTTTTTTTT508250.48062747394304034No Hit
TATTTTTTTTTTTTTTTTTTTTTTA467510.4421016238920035No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT455240.4304984775953364No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC452470.4278790224004082No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT450820.4263186971037904No Hit
TTTTTTTATTTTTTTTTTTTTTTTT415430.392852083620575No Hit
TTTTTATTTTTTTTTTTTTTTTTTA412290.38988273729852657No Hit
TTTTTTTTTTTTTTTTTTTTTTATT384430.36353688107805804No Hit
TTTTTTTTATTTTTTTTTTTTTTTT360290.3407088491626916No Hit
TTTATTTTTTTTTTTTTTTTTTTTA344460.32573918283210956No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC297560.28138811834036614No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG286050.27050366733183806No Hit
TTATTTTTTTTTTTTTTTTTTTTTA276320.26130247634026743No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA265170.25075845994191054No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG253050.23929716139948126No Hit
TTTTATTTTTTTTTTTTTTTTTTTA247530.2340771640435234No Hit
TTTTTTATTTTTTTTTTTTTTTTTA231430.218852171755313No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA228780.21634619476377528No Hit
TTTTTTTTTTTTTTTTTTTTTAATT227570.21520195621292218No Hit
TATTTTTTTTTTTTTTTTTTTTTAA218890.20699369950101743No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC213020.2014427240518376No Hit
TTTTTTTATTTTTTTTTTTTTTTTA196800.18610425356023677No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC186760.17660991054324096No Hit
TTTTTATTTTTTTTTTTTTTTTTAA183330.1733663252296657No Hit
TTTTTTTTTATTTTTTTTTTTTTTT170510.16124307050079256No Hit
TTTTTATTTTTTTTTTTTTTTTTAT169530.16031633183977104No Hit
TTTTTTTTATTTTTTTTTTTTTTTA168100.15896404991603558No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC165080.15610818179737748No Hit
TATTTTTTTTTTTTTTTTTTTTTAT164010.1550963344838132No Hit
TATATTTTTTTTTTTTTTTTTTTTT162490.15365894390753493No Hit
TATTTATTTTTTTTTTTTTTTTTTT159360.15069905410243564No Hit
TTTATATTTTTTTTTTTTTTTTTTT156070.14758786002614918No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA150680.1424907973905309No Hit
TTTATTTTTTTTTTTTTTTTTTTAA149500.1413749283905254No Hit
TTTATTTTTTTTTTTTTTTTTTTAT138140.13063232513623532No Hit
AATTTTTTTTTTTTTTTTTTTTTTT136860.12942189096673784No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT135070.12772917443283122No Hit
TTTTTTTTTTATTTTTTTTTTTTTT133380.1261310230684166No Hit
TTATTTTTTTTTTTTTTTTTTTTAA127580.12064624323788115No Hit
TTATTATTTTTTTTTTTTTTTTTTT124920.1181308097293942No Hit
TTTTTTTTTTTATTTTTTTTTTTTT119010.11254200821241758No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA113050.10690592411069495No Hit
TATTATTTTTTTTTTTTTTTTTTTT110130.10414462116152885No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA109890.10391766475474806No Hit
TTATTTTTTTTTTTTTTTTTTTTAT109410.10346375194118652No Hit
TTTTATTTTTTTTTTTTTTTTTTAA106540.1007497315767664No Hit
TTTTTATATTTTTTTTTTTTTTTTT106380.1005984273055792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)