FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_AKR1C4_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_AKR1C4_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9259705
Sequences flagged as poor quality0
Sequence length25
%GC4

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT7953278.58911811985371No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4058884.383379384116449No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1758701.8993045674781217No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1573361.6991470030632723No Hit
TATTTTTTTTTTTTTTTTTTTTTTT806910.8714208498002906No Hit
TTTTTATTTTTTTTTTTTTTTTTTT748820.8086866698237147No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG741930.8012458280258388No Hit
TTTATTTTTTTTTTTTTTTTTTTTT664860.718014234794737No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA663100.7161135262948441No Hit
TTATTTTTTTTTTTTTTTTTTTTTT537890.5808932357996286No Hit
TTTTATTTTTTTTTTTTTTTTTTTT473980.5118737583972708No Hit
TTTTTTATTTTTTTTTTTTTTTTTT439430.4745615546067612No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT422310.4560728446532584No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT405180.43757333521964253No Hit
TATTTTTTTTTTTTTTTTTTTTTTA399200.43111524611205215No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC382710.41330690340567006No Hit
TTTTTTTATTTTTTTTTTTTTTTTT365260.3944618106084373No Hit
TTTTTTTTTTTTTTTTTTTTTTATT363210.3922479171852667No Hit
TTTTTATTTTTTTTTTTTTTTTTTA353220.38145923655235237No Hit
TTTATTTTTTTTTTTTTTTTTTTTA315150.3403456157620572No Hit
TTTTTTTTATTTTTTTTTTTTTTTT311470.33637140708046315No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC275220.29722329167073896No Hit
TTATTTTTTTTTTTTTTTTTTTTTA262040.2829895768817689No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG231980.25052633966200866No Hit
TTTTATTTTTTTTTTTTTTTTTTTA226990.245137399085608No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA218900.2364006196741689No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG214700.2318648380266974No Hit
TTTTTTTTTTTTTTTTTTTTTAATT208480.22514756139639436No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA206820.22335484769763184No Hit
TTTTTTATTTTTTTTTTTTTTTTTA202000.21814949828315267No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC184520.19927200704558082No Hit
TATTTTTTTTTTTTTTTTTTTTTAA180380.19480102227878748No Hit
TTTTTTTATTTTTTTTTTTTTTTTA176190.19027604011142904No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC157240.1698110252972422No Hit
TTTTTATTTTTTTTTTTTTTTTTAT153970.16627959530028225No Hit
TATTTTTTTTTTTTTTTTTTTTTAT153640.16592321245655234No Hit
TTTTTATTTTTTTTTTTTTTTTTAA151100.1631801445078434No Hit
TTTTTTTTTATTTTTTTTTTTTTTT151070.16314774606750432No Hit
TTTTTTTTATTTTTTTTTTTTTTTA146680.1584067742978853No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA146610.15833117793709411No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC145400.1570244408434178No Hit
TATATTTTTTTTTTTTTTTTTTTTT141230.15252105763628537No Hit
TTTATATTTTTTTTTTTTTTTTTTT138580.14965919540633313No Hit
TTTATTTTTTTTTTTTTTTTTTTAT133780.14447544495208No Hit
TTTATTTTTTTTTTTTTTTTTTTAA133040.143676283423716No Hit
TATTTATTTTTTTTTTTTTTTTTTT129680.1400476581057388No Hit
TTTTTTTTTTATTTTTTTTTTTTTT122140.13190485010051617No Hit
AATTTTTTTTTTTTTTTTTTTTTTT118370.12783344609790484No Hit
TTATTTTTTTTTTTTTTTTTTTTAA114200.12333006289077245No Hit
TTATTTTTTTTTTTTTTTTTTTTAT111800.12073818766364587No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT111750.12068419026308073No Hit
TTATTATTTTTTTTTTTTTTTTTTT110790.11964744017223011No Hit
TTTTTTTTTTTATTTTTTTTTTTTT106650.11517645540543679No Hit
TTTTATTTTTTTTTTTTTTTTTTAT99330.10727123596270076No Hit
TATTATTTTTTTTTTTTTTTTTTTT97520.1053165300622428No Hit
TTTTATTTTTTTTTTTTTTTTTTAA96340.10404219140890557No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA96240.1039341966077753No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA95000.1025950610737599No Hit
TAATTTTTTTTTTTTTTTTTTTTTT92760.10017597752844179No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)