FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_AKR1C4_no_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_AKR1C4_no_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11166031
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT10757419.634049914423487No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA5038394.512247906171853No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2269292.0323156903289985No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1799371.6114678528118005No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1135431.016860870259092No Hit
TTTTTATTTTTTTTTTTTTTTTTTT961470.8610669270038744No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG960700.8603773355098154No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA892860.7996216381631038No Hit
TTTATTTTTTTTTTTTTTTTTTTTT848420.7598223576488369No Hit
TTATTTTTTTTTTTTTTTTTTTTTT678730.6078525126788561No Hit
TTTTATTTTTTTTTTTTTTTTTTTT599070.5365111381116531No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT559280.500876273762808No Hit
TTTTTTATTTTTTTTTTTTTTTTTT535200.4793108670395058No Hit
TATTTTTTTTTTTTTTTTTTTTTTA519190.46497273740329037No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC509170.45599909224683327No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT470100.4210090407236018No Hit
TTTTTTTATTTTTTTTTTTTTTTTT457380.40961734747109335No Hit
TTTTTTTTTTTTTTTTTTTTTTATT415470.37208386758016343No Hit
TTTTTATTTTTTTTTTTTTTTTTTA413180.37003300456536437No Hit
TTTTTTTTATTTTTTTTTTTTTTTT378530.3390013873327058No Hit
TTTATTTTTTTTTTTTTTTTTTTTA364770.3266782977765331No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC346040.3099042085768882No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG339250.3038232654020036No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA306730.2746992194451188No Hit
TTATTTTTTTTTTTTTTTTTTTTTA300430.2690571072209991No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG281640.25222928361921976No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA267670.23971812365557646No Hit
TTTTATTTTTTTTTTTTTTTTTTTA256210.22945485284789194No Hit
TATTTTTTTTTTTTTTTTTTTTTAA241730.21648695046610564No Hit
TTTTTTTTTTTTTTTTTTTTTAATT238120.21325393060434816No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC237130.21236731296912933No Hit
TTTTTTATTTTTTTTTTTTTTTTTA224570.20111891145564614No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC210980.18894806937218783No Hit
TTTTTTTATTTTTTTTTTTTTTTTA195560.17513832802362808No Hit
TATATTTTTTTTTTTTTTTTTTTTT193750.1735173402259048No Hit
TTTTTATTTTTTTTTTTTTTTTTAA183570.16440040333042244No Hit
TATTTTTTTTTTTTTTTTTTTTTAT182180.16315555634764045No Hit
TTTTTTTTTATTTTTTTTTTTTTTT181570.1626092565926066No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA181470.16251969925571583No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC181110.162197292842909No Hit
TATTTATTTTTTTTTTTTTTTTTTT179420.16068377384945465No Hit
AATTTTTTTTTTTTTTTTTTTTTTT173250.15515808616329294No Hit
TTTATATTTTTTTTTTTTTTTTTTT173080.1550058386905786No Hit
TTTTTATTTTTTTTTTTTTTTTTAT170270.15248927752394742No Hit
TTTTTTTTATTTTTTTTTTTTTTTA160920.14411566652465857No Hit
TTTATTTTTTTTTTTTTTTTTTTAA158600.14203793630879227No Hit
TTTTTTTTTTATTTTTTTTTTTTTT145670.13045817264881318No Hit
TTTATTTTTTTTTTTTTTTTTTTAT145170.13001038596435924No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT140210.12556834205457607No Hit
TTATTATTTTTTTTTTTTTTTTTTT137050.12273833020882711No Hit
TTATTTTTTTTTTTTTTTTTTTTAA136340.1221024731169025No Hit
TTTTTTTTTTTATTTTTTTTTTTTT129310.11580659233348No Hit
TATTATTTTTTTTTTTTTTTTTTTT128760.11531402698058066No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA128670.11523342537737893No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA123860.11092571747293198No Hit
TAATTTTTTTTTTTTTTTTTTTTTT122240.10947488861530119No Hit
TTATTTTTTTTTTTTTTTTTTTTAT119220.10677025704119933No Hit
TTTTTTTTTTTTTTTTTTTTTTACC115980.10386859932593774No Hit
TTTTTTTTTTTTTTTTTTTTAAAAA115110.10308945049498788No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG112700.10093111867591985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)