FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_AKR1C4_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_AKR1C4_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10506330
Sequences flagged as poor quality0
Sequence length25
%GC12

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT8477038.068497753259225No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3700233.521905365622439No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1591801.5150866192095622No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1456651.3864498830704917No Hit
TATTTTTTTTTTTTTTTTTTTTTTT906930.8632224573185879No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG890220.8473177598647673No Hit
TTTTTATTTTTTTTTTTTTTTTTTT794280.7560013820239798No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG705810.6717950035835539No Hit
TTTATTTTTTTTTTTTTTTTTTTTT647490.6162856106747076No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA626800.5965927207692886No Hit
TTATTTTTTTTTTTTTTTTTTTTTT528760.5032775479163514No Hit
TTTTATTTTTTTTTTTTTTTTTTTT473090.4502904439514083No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT445940.4244488798657571No Hit
TTTTTTATTTTTTTTTTTTTTTTTT444380.4229640607138744No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT382930.36447551142977613No Hit
TATTTTTTTTTTTTTTTTTTTTTTA382680.36423755964261545No Hit
TTTTTTTATTTTTTTTTTTTTTTTT363720.3461912961043485No Hit
TTTTTTTTTTTTTTTTTTTTTTATT344410.3278119000640566No Hit
TTTTTATTTTTTTTTTTTTTTTTTA318110.30277937205475175No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC316490.30123744447395046No Hit
TTTTTTTTATTTTTTTTTTTTTTTT312220.29717322794924583No Hit
TTTATTTTTTTTTTTTTTTTTTTTA258610.2461468467105069No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC256220.24387202762525068No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA221390.21072058463802298No Hit
TTATTTTTTTTTTTTTTTTTTTTTA216580.20614239225305125No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG214520.20418166952684713No Hit
TTTTTTTTTTTTTTTTTTTTTAATT210970.20080275414916532No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA190220.18105275581482783No Hit
TTTTATTTTTTTTTTTTTTTTTTTA186330.17735022600660746No Hit
GCCCCCCCCCCCCCCCCCCCCCCCC176090.16760372080450547No Hit
TATTTTTTTTTTTTTTTTTTTTTAA169550.16137890205238176No Hit
TTTTTTATTTTTTTTTTTTTTTTTA168860.16072215511981824No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC166800.15876143239361415No Hit
GCCCCCCCCCCCCCCCCCCCCCGGC162850.1550017941564752No Hit
TTTTTTTTTATTTTTTTTTTTTTTT156440.14890071033367502No Hit
TATTTATTTTTTTTTTTTTTTTTTT152260.14492215645234827No Hit
TATTTTTTTTTTTTTTTTTTTTTAT150300.14305661444100842No Hit
TATATTTTTTTTTTTTTTTTTTTTT149440.14223806029317565No Hit
GCCCCCCCCCCCCCCCCCCCCCCGC149060.1418763735766914No Hit
TTTTTTTATTTTTTTTTTTTTTTTA148980.14180022900479997No Hit
AATTTTTTTTTTTTTTTTTTTTTTT142870.13598468732659263No Hit
TTTTTATTTTTTTTTTTTTTTTTAT142410.13554685603821695No Hit
TTTATATTTTTTTTTTTTTTTTTTT139850.1331102297376915No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA137810.1311685431544602No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC135540.12900794092704113No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC132440.12605733876624853No Hit
TTTTTATTTTTTTTTTTTTTTTTAA129990.12372541125207374No Hit
TTTTTTTTATTTTTTTTTTTTTTTA120590.11477842405483171No Hit
TTTTTTTTTTATTTTTTTTTTTTTT119390.11363625547646038No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT115970.11038107502810211No Hit
TTTATTTTTTTTTTTTTTTTTTTAT113410.10794444872757661No Hit
TTATTATTTTTTTTTTTTTTTTTTT112840.10740191865285023No Hit
TTTTTTTTTTTATTTTTTTTTTTTT107580.10239541305098927No Hit
TTTATTTTTTTTTTTTTTTTTTTAA107220.10205276247747785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)