FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_AKR1C4_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_AKR1C4_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11097442
Sequences flagged as poor quality0
Sequence length25
%GC11

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT9843698.870233338457638No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4427923.9900366228541677No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1996211.7988019221006064No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1446711.3036427674053175No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1072950.9668444313563432No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG980050.8831314459674581No Hit
TTTTTATTTTTTTTTTTTTTTTTTT889800.8018063982672763No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG776350.6995756319339177No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA770070.6939166701659716No Hit
TTTATTTTTTTTTTTTTTTTTTTTT717940.6469418808406477No Hit
TTATTTTTTTTTTTTTTTTTTTTTT585470.5275720296623312No Hit
TTTTATTTTTTTTTTTTTTTTTTTT532000.4793897548642291No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT525680.4736947487538119No Hit
TTTTTTATTTTTTTTTTTTTTTTTT496670.44755358937672307No Hit
TATTTTTTTTTTTTTTTTTTTTTTA471930.4252601635584129No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC432510.3897384640532476No Hit
TTTTTTTATTTTTTTTTTTTTTTTT416080.3749332503832865No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT380360.34274565255668826No Hit
TTTTTATTTTTTTTTTTTTTTTTTA375510.3383752760320802No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC356610.32134432421453524No Hit
TTTTTTTTATTTTTTTTTTTTTTTT339160.30561997981156375No Hit
TTTTTTTTTTTTTTTTTTTTTTATT314710.28358787547616826No Hit
TTTATTTTTTTTTTTTTTTTTTTTA300270.2705758678441392No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG289300.26069070692146895No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA268710.2421368816345244No Hit
TTATTTTTTTTTTTTTTTTTTTTTA255290.23004400473550574No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA232650.20964290689692275No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC224800.20256920468699002No Hit
TTTTATTTTTTTTTTTTTTTTTTTA223110.2010463312175905No Hit
TATTTTTTTTTTTTTTTTTTTTTAA219740.1980095953644092No Hit
TTTTTTATTTTTTTTTTTTTTTTTA204750.18450197802340396No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA192060.1730669103744809No Hit
TTTTTTTTTTTTTTTTTTTTTAATT191340.172418112210003No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC179960.162163496777005No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC176270.15883840618405573No Hit
TTTTTTTATTTTTTTTTTTTTTTTA174350.157108277745448No Hit
TATTTATTTTTTTTTTTTTTTTTTT173260.15612606941311338No Hit
TTTTTTTTTATTTTTTTTTTTTTTT173070.1559548587863762No Hit
AATTTTTTTTTTTTTTTTTTTTTTT168000.1513862383781776No Hit
TATATTTTTTTTTTTTTTTTTTTTT166960.15044908547393174No Hit
TTTTTATTTTTTTTTTTTTTTTTAA166870.150367985703372No Hit
TATTTTTTTTTTTTTTTTTTTTTAT149130.13438231981748588No Hit
TTTATATTTTTTTTTTTTTTTTTTT148620.1339227544509807No Hit
TTTTTTTTATTTTTTTTTTTTTTTA143410.12922797884413362No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT136570.12306439628159355No Hit
TTTTTATTTTTTTTTTTTTTTTTAT135150.12178482212387323No Hit
TTTATTTTTTTTTTTTTTTTTTTAA132240.11916259620910837No Hit
GCCCCCCCCCCCCCCCCCCCCCCCC131620.11860390890080796No Hit
TTTTTTTTTTATTTTTTTTTTTTTT128900.1161528936127803No Hit
TTATTATTTTTTTTTTTTTTTTTTT120630.10870072580690217No Hit
GCCCCCCCCCCCCCCCCCCCCCGGC118380.10667323154290871No Hit
TTTTTTTTTTTATTTTTTTTTTTTT117190.10560091235439661No Hit
TATTATTTTTTTTTTTTTTTTTTTT116430.10491606984744772No Hit
TTTTTTTTTTTTTTTTTTTTAAAAA113660.10241999913133135No Hit
TTATTTTTTTTTTTTTTTTTTTTAA112850.10169010119629371No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA111350.1003384383536314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)