FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_NGFR_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_NGFR_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8177154
Sequences flagged as poor quality0
Sequence length50
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACTCTATCCGATCTCGTATGCC775090.9478725727802118No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC230130.28143043410947133No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTCTATCCGATCTCGTATGC162970.199299169368707No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA131730.16109516831895304No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTATCCGATCTCGTATGGC121860.14902495415886752No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110910.13563398708156899No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT103560.12664552972831378No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA100500.12290339646287694No Hit
CTTAGCTGAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87530.10704213226264297No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA83650.10229720511561846No Hit
TCAATAAAGTACCCTGTGCTCAACCAAAAAAAAAAAAAAAAAAAAAAAAA81850.1000959502535968No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)