FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_NGFR_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_NGFR_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10408079
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCC915640.8797396714609872No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC352260.3384486224595336No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGC202670.19472373336136284No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGGC169790.16313288936411802No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA147840.1420435029365169No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG124000.11913821945432966No Hit
CTTAGCTGAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA122370.11757212834376066No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT117720.11310444511422328No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA116030.11148070647811187No Hit
GCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGA105470.10133474198264637No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105380.10124827069433273No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)