FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_NGFR_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_NGFR_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9061211
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC233190.2573497074508032No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178920.1974570507187174No Hit
GCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGA153610.16952480192768934No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTATGCC124860.13779615108841411No Hit
GGCATGGTTGTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAA103640.11437764775591253No Hit
CCCTCTACACTTATCATCTTCACAATTCTAGTTCTACTGACTATCCTAGA101630.11215940121027973No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100710.11114408438342292No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTATGGC92210.10176343978746329No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)