FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_NGFR_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_NGFR_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14004180
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC436650.3117997626422968No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTATGGC262730.18760827124472834No Hit
GCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGA216990.15494659451677997No Hit
GCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCA211570.1510763214982955No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGGGTGGC207330.14804865404472095No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT200080.14287162832811345No Hit
GGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCATTTTTTTTTT188200.13438844687800355No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTGGGGTGGC176450.12599809485453628No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTGTGGC157890.11274490902002117No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG151330.1080605933371322No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCGGGGGTGGC147390.10524714763734827No Hit
GGCATGGTTGTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAA146670.10473301542825071No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGGATGGC140790.10053426905395389No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)