FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_LTBR_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_LTBR_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12076611
Sequences flagged as poor quality0
Sequence length50
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGCTGAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310320.25695950627208247No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC272720.22582494376940684No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTATGGC255570.21162393986193642No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA226440.18750293439111354No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGGGTGGC217860.180398292203003No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA201480.16683488438933736No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTGGGGTGGC190880.15805758751358306No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTGTGGC162060.13419327657403224No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCGGGGGTGGC156600.12967214063614368No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGGATGGC146020.12091140469789083No Hit
CCACTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTT140060.11597624532246671No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA128920.10675180313417398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)