FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_LTBR_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_LTBR_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6726629
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCGGGGGGGGG503890.7490973561943137No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCGGGGGTGGG298790.444189801459245No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC257500.3828069007522193No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATGGGGGGGGGG242860.3610426559871222No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCGGGGGGGG142930.21248384592044545No Hit
GCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGA106620.15850435634253054No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATGGGGGGTGGG102750.1527511031156914No Hit
GCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCA97320.1446787090532271No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCGGGGGTGGC95730.14231496935537846No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG89470.13300867343806236No Hit
GGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCATTTTTTTTTT86260.1282365951801415No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCGGGGGGGGC77390.11505019824937573No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATGGGGGGGGG76700.11402442441823385No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT70790.10523844856019263No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)