FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_LTBR_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_LTBR_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13754632
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACGTCTACCAATCTCGTATGCC602740.4382087430619736No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC512870.3728707536486618No Hit
GCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCA237400.17259640243374014No Hit
GCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGA225190.16371939285616657No Hit
GGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCATTTTTTTTTT213440.1551768160718513No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTACCAATCTCGTATGGC203970.1482918626975989No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG176430.12826951677078674No Hit
CCCTCTACACTTATCATCTTCACAATTCTAGTTCTACTGACTATCCTAGA172540.1254413785843198No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT161980.11776396489560753No Hit
CCCTGGGAGTGGGTGGAGGCAGCCAGGGCTTACCTGTACACTGACTTGAG159090.11566285452057169No Hit
CCAAGATGGCGGCCGCAAACTGACACCTCAGGGACAAAGAGATCTGGACA142950.10392862564407394No Hit
AGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTC139570.10147127164143686No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)