FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_IL12B_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_IL12B_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13432916
Sequences flagged as poor quality0
Sequence length50
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCGGGGGGGGG795220.5919935775672237No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCGGGGGTGGG478580.35627409566173124No Hit
CTTAGCTGAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA405770.30207141919148456No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATGGGGGGGGGG389930.2902794895762022No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC320220.23838457710894642No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCGGGGGGGG227820.16959832101979944No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA196660.14640157058973644No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATGGGGGGTGGG161620.12031639295593005No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA159430.11868606935381715No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT159290.11858184775368208No Hit
CCCTATGGGGATGGTCCACTGTCACTGTTTCTCTGCTGTTGCAAATACAT153370.11417476294797049No Hit
GCCACTAAACTGGGTTAAATGTACACTGTTGAGTTTTCTGTACATAAAAA150770.11223921894546202No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCGGGGGTGGC145170.10807035494005918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)