FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_IL12B_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_IL12B_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13611723
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCC1089650.800523196071504No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGC254780.18717689156618894No Hit
GCTACTCGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAG211140.15511629203738572No Hit
GCTACTTGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAG201130.14776233692090265No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGGC195160.14337641164164155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)