FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_IL12B_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_IL12B_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9372535
Sequences flagged as poor quality0
Sequence length50
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGCC1701361.815261292702561No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGC382170.40775521243719015No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351430.37495725542769376No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA316660.33785950119151326No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGGC316640.33783816224746027No Hit
GTGTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128470.1370707071245933No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTGTGCC124300.132621537289538No Hit
GGCATGGTTGTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAA123450.13171463216728452No Hit
GTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAATCATAAAAC118570.1265079298183469No Hit
GTCATGTAGGTTGTCTAAAAATAAAATGCATTTAAACTCAAAAAAAAAAA117430.12529161000732458No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC115360.12308302929783671No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATGTCGTATGCC100100.10680141498538016No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA95410.10179743260494627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)