FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_IFNL2_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_IFNL2_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11459780
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCC543650.47439828687810764No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC400770.3497187555083955No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT155760.13591883962868395No Hit
CCCTCTACACTTATCATCTTCACAATTCTAGTTCTACTGACTATCCTAGA143850.12552596995753845No Hit
GGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCATTTTTTTTTT136240.11888535382005588No Hit
CCAACTTAGCCAGTTTTATTTGCAAAACAAGGAAATAAAGGCTTACTTCT134160.11707031025028404No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG133260.11628495485951737No Hit
GCCACTAAACTGGGTTAAATGTACACTGTTGAGTTTTCTGTACATAAAAA127500.11125868035861072No Hit
AGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTC126960.11078746712415073No Hit
CTTAGCTGAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA122930.10727082020771778No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGC120130.1048274923253326No Hit
AAAGATAAAAGGTTTCTTTTTTTTTCCTTTTTTGTCTATGAAGTTGCTGT116810.10193040355050446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)