FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_IFNL2_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_IFNL2_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10156453
Sequences flagged as poor quality0
Sequence length50
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGCC2100992.0686257298684887No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA573380.5645474852293414No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT493460.48585859650017577No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGC436160.4294412626140248No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGGC396230.3901263561205866No Hit
GTGTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183270.18044685482224945No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTGTGCC154710.1523268014926077No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA137510.1353917553697142No Hit
ACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132910.13086261512754502No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATGTCGTATGCC126800.12484673537109856No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTGTGGC115470.11369126603549488No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA114920.11314973839784422No Hit
AAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCATCC112160.11043225425254269No Hit
CTACCTGCACGACAACACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107550.1058932680533253No Hit
GGCATGGTTGTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAA103730.10213211246091523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)