FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_IFNL2_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_IFNL2_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10835239
Sequences flagged as poor quality0
Sequence length50
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT641340.591902033725329No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA556190.5133158576382117No Hit
GTGTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237370.21907223273985926No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTATGCC195250.18019907082806388No Hit
ACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158680.1464480848092045No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTATGGC153640.1417965953496734No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA134520.12415046866986507No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA116200.10724267365029973No Hit
GTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAATCATAAAAC109420.10098531282974008No Hit
CTCAAAATTCTGTGACAAATTTTTGAGACCCATTAAAAAAGTTAAATGAG108450.10009008569169539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)