FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_IFNL2_no_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_IFNL2_no_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10018662
Sequences flagged as poor quality0
Sequence length50
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTATGCC443640.44281362122007906No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT412260.41149207349244843No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTATGGC336660.3360328954105848No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA331930.3313117060940872No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATGTCGTATGGC213620.21322208494507552No Hit
GTGTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165570.16526158882293865No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATGTCGTGTGGC144050.1437816746387891No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTGTGGC139560.13930003826858317No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATGTCGTATGCC132300.13205356164326135No Hit
GTCATGTAGGTTGTCTAAAAATAAAATGCATTTAAACTCAAAAAAAAAAA118510.11828924860425474No Hit
GGCATGGTTGTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAA117720.11750072015604479No Hit
GTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAATCATAAAAC113980.11376768674300022No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTATGC112650.1124401641656341No Hit
ACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111240.11103279060617076No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATGGGGGGTGGC107860.10765908661256364No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA105440.10524359440412302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)