FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_IFNL2_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_IFNL2_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12635854
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTATGGC343540.2718771521101779No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC334600.2648020466206716No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGGGTGGC258760.2047823597835176No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTGGGGTGGC212020.16779237873435385No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTGTGGC206560.16347134115351444No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGGATGGC188450.14913910844490605No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCGGGGGTGGC168020.1329708304638531No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTATGCC156250.12365606630149414No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150240.11889975936727347No Hit
GGCATGGTTGTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAA148470.11749898344821015No Hit
GTCATGTAGGTTGTCTAAAAATAAAATGCATTTAAACTCAAAAAAAAAAA148400.11744358553050709No Hit
GCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCA141840.11225200924290515No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT140210.11096202915924797No Hit
GCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGA135060.10688632521395072No Hit
GTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAATCATAAAAC127920.10123573760823763No Hit
GGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCATTTTTTTTTT127190.10065801646647705No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)