FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_IFNL2_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_IFNL2_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10246727
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCC807420.7879784442388287No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC327060.3191848480007323No Hit
GCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGA176250.172006144010668No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGC148930.1453439717872839No Hit
CCCTCTACACTTATCATCTTCACAATTCTAGTTCTACTGACTATCCTAGA136870.1335743598907241No Hit
GCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCA135340.13208120017250388No Hit
AGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTC134020.1307929839450197No Hit
CCAAGATGGCGGCCGCAAACTGACACCTCAGGGACAAAGAGATCTGGACA117820.11498305751680513No Hit
GGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCATTTTTTTTTT116040.11324591745247042No Hit
AAAGATAAAAGGTTTCTTTTTTTTTCCTTTTTTGTCTATGAAGTTGCTGT113660.11092322455746113No Hit
CCAGTAAGCTACCCTTTTACCATCATTGGACAAGTAGCATCCGTACTATA109330.10669748496275934No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG106670.10410153408010187No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT105810.10326224168946826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)