FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_DUPD1_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_DUPD1_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12591184
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGCTGAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA272150.2161432951817716No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC265820.21111596812499922No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTATGGC236850.18810780622378326No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA191340.1519634690431019No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGGGTGGC177060.14062220042213663No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTGGGGTGGC149650.11885300063917738No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT149340.11860679662849816No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTGTGGC138190.10975139430890693No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGGATGGC130310.10349304719873842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)