FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_DUPD1_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_DUPD1_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9768904
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCC1923231.9687264814967984No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGC396950.4063403632587647No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC214760.21984042426867947No Hit
CTTAGCTGAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA199200.20391233243770235No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATGTCGTATGCC190450.19495533992349603No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGCTCTCGTATGCC187890.19233477982791108No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA137180.14042516949700806No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCCGT124460.12740426152207043No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT116720.11948116185807538No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100210.10258059655412727No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGAGCTCGTATGCC97910.10022618709325018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)