FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_DUPD1_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_DUPD1_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11731428
Sequences flagged as poor quality0
Sequence length50
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCGGGGGGGGG607410.517763054932443No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC408190.3479457061834246No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCGGGGGTGGG389170.3317328461633145No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATGGGGGGGGGG282920.24116416177126943No Hit
CTTAGCTGAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230870.19679616155850763No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCGGGGGGGG167410.1427021501559742No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA155400.13246469227787103No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA150570.12834754643680207No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG144120.12284949453723792No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATGGGGGGTGGG125360.10685826141540483No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT120320.10256210923341985No Hit
GCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCA119680.10201656609919953No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCGGGGGTGGC118390.10091695571928667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)