FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_DUPD1_no_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_DUPD1_no_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9639153
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCGGGGGGGGG248180.2574707549511871No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC239500.2484658143718644No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCGGGGGTGGG159100.16505599610256214No Hit
GCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGA135820.14090449648428652No Hit
AGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTC126490.13122522279706528No Hit
CCCTCTACACTTATCATCTTCACAATTCTAGTTCTACTGACTATCCTAGA119680.1241602866973893No Hit
GCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCA116870.12124509280016615No Hit
CCAGTAAGCTACCCTTTTACCATCATTGGACAAGTAGCATCCGTACTATA114780.11907685249938453No Hit
CCAAGATGGCGGCCGCAAACTGACACCTCAGGGACAAAGAGATCTGGACA113190.11742732997390953No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATGGGGGGGGGG113140.11737545819637887No Hit
GTAAAGTGGTTGGTTGCAGTTGTGTAGTAGTTAAGGACTATGGCAAGGAG107040.11104710133763827No Hit
GGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCATTTTTTTTTT106120.1100926606310741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)