FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_DUPD1_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_DUPD1_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11919939
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACGTCTACCAATCTCGTATGCC464050.3893056835274073No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC410310.34422156019422584No Hit
GCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGA207680.17422907952800765No Hit
GCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCA157530.13215671657380126No Hit
CCCTCTACACTTATCATCTTCACAATTCTAGTTCTACTGACTATCCTAGA154830.12989160431106234No Hit
GGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCATTTTTTTTTT154700.1297825433502638No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTACCAATCTCGTATGGC146570.12296203864801657No Hit
CCAAGATGGCGGCCGCAAACTGACACCTCAGGGACAAAGAGATCTGGACA144440.12117511675185585No Hit
AGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTC136660.11464823771329702No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG134190.11257607945812476No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT133750.11220695005234507No Hit
CCAGTAAGCTACCCTTTTACCATCATTGGACAAGTAGCATCCGTACTATA125790.10552906352960362No Hit
CCCTGGGAGTGGGTGGAGGCAGCCAGGGCTTACCTGTACACTGACTTGAG119680.10040319837207222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)