FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_AKR1C4_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_AKR1C4_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9259705
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCC490810.5300492834274958No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC271770.29349747103174456No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT136000.14687292953717207No Hit
CTTAGCTGAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA118260.12771465181666153No Hit
CCCTCTACACTTATCATCTTCACAATTCTAGTTCTACTGACTATCCTAGA116510.12582474279688177No Hit
AGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTC113910.12301687796749464No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGC103790.11208780409311095No Hit
CCAACTTAGCCAGTTTTATTTGCAAAACAAGGAAATAAAGGCTTACTTCT103330.1115910280079117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)