FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_AKR1C4_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_AKR1C4_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10506330
Sequences flagged as poor quality0
Sequence length50
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGCC2751132.6185452008455856No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGC608030.5787273005892638No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGGC480060.45692453977744846No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA373460.3554618977321291No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355120.33800575462602067No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTGTGCC182520.17372384077027847No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATGTCGTATGCC165150.15719095059835356No Hit
GTGTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134320.12784673620569695No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTGTGGC123880.11790986957386641No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGCCGT117440.11178023153660699No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA109950.10465119599327263No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)