FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n01_AKR1C4_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n01_AKR1C4_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11097442
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT521610.4700272369073882No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTATGCC416180.375023361239464No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTATGGC300560.2708371893270539No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA229620.2069125479547449No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATGTCGTATGGC196600.17715794324493878No Hit
GTGTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177570.16000984731436307No Hit
ACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134470.12117206830186632No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC130910.1179641218219478No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATGTCGTATGCC129150.11637817075322403No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA127180.11460298688652755No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATGTCGTGTGGC122770.11062909812910038No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTGTGGC118680.10694356411144118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)