FastQCFastQC Report
Sat 5 Jun 2021
H2Y5VBGXJ_n01_S11_R2_G11_fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y5VBGXJ_n01_S11_R2_G11_fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences676723
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTACCCATTGTCAAACAATGTATCAGACAACACAGGGTCTTG16180.23909339567297105No Hit
GTATAAATATAATTACCCATTGTCATAAAATCTTGTCTTTAAATCAGTTT13410.19816084276727702No Hit
TCTCTACACAGTGCCGAGATGAAGGGCATCAAGATCAAGGTACAGGCCCC11650.1721531557225039No Hit
CATATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCA10740.15870599935276325No Hit
CTATAGACCAGGTAGACCTTTGAACCACATCTGAGGTTTCTATTTCCTGT10440.15427287087922237No Hit
CCCTAGGCCTGGACACACAGCCAAGTCCCTGCAAGCCTTTGGTCCCCACC10410.1538295580318683No Hit
GTGTAGAGAGGTTCAAGCGGGGATGGATTCTCCCCTGAGCTCGCTTCTTG10370.15323847423539616No Hit
GTGGTATGGGGAAGCTGTGATCTGAGCCAGGCCTGGGGAAGTTCCTCTGG9780.14451998823743245No Hit
GTATAGCACCCGTGGTGTTTTGAGCCTAGTTCTGTGTGCTAGAGAGGCAG9670.1428945077971341No Hit
GTTAGTGACCATCACTGTGGGCCCAGCACTGCGTTCCGGGCTACAGGCTA9430.13934800501830144No Hit
ATCTAAGACCACAGTGCTCAAGGGAAGTCAGAAAGGGGACTCAGACCGAT8580.12678747434326895No Hit
CATCTGAGGTTTCTATTTCCTGTTCCGTCATTCACCGGCTTGTGTGACCT8560.1264919324450329No Hit
ATCCTAAAGGTGCCAAATTTATCTCCAGATAAATTTGGAAGCAACTGTGG8460.12501422295385262No Hit
GTGCTAGGCCCCAGGCTTCCTGTGTGTAGCTCGGAACCCTGCCCAGGGAC8190.12102440732766583No Hit
TAATTACCCATTGTCATAAAATCTTGTCTTTAAATCAGTTTTGAGAACCG8090.11954669783648553No Hit
TATTTATACTTCTCCACAGTAAAATTAACTTGGGGAAATTTTTTCCTCTT7970.11777344644706919No Hit
GTGTGTGTGTGTTAAGCATGGAACACCACAAACCAGGTATTTTTTGCATG7660.11319254702441027No Hit
GCCCAGGGACTGCCCACTGAGCAGTCGTTCAGAGAGGGAGCCAAGAATCT7610.11245369227882013No Hit
GCTCAAGGGAAGTCAGAAAGGGGACTCAGACCGATCATCTGGGTGTCTGC7540.11141929563499393No Hit
GGTATGGGGAAGCTGTGATCTGAGCCAGGCCTGGGGAAGTTCCTCTGGCA7420.10964604424557758No Hit
GTGTTTGAACAGTGGTATGGGGAAGCTGTGATCTGAGCCAGGCCTGGGGA7350.10861164760175139No Hit
CTCTAGGAATTCATAAAAGCATAAGAGGCAGCAAAATACAGAGGCAGATT7290.1077250219070432No Hit
GTCTAATATCTGAAGAATGACAGATTCACGCACATATACACAGAATGTGG7180.1060995414667449No Hit
CCTTAGGGAAGGGATTGGCCACCGGCATCCGGGCATCCCGCAGCTCTTCG7010.1035874353317384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)