FastQCFastQC Report
Sat 5 Jun 2021
H2Y5VBGXJ_n01_S11_R2_D10_fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y5VBGXJ_n01_S11_R2_D10_fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488051
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA29030.5948148861491934No Hit
CTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCT23900.48970292039151647No Hit
GGATTTATGTGCAACTGATCCCCTCAGAATGAGTGCTGACCGTGCCAGGA23800.48765395419740964No Hit
CCCATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTA23260.47658953674923316No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA22880.46880346521162747No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC21610.4427815945464716No Hit
CCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTCGGTCATTGATT21520.4409375249717755No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA21110.43253676357593773No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT21100.4323318669565271No Hit
ATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATGACA20440.41880869007542243No Hit
TCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCAC20360.41716951712013706No Hit
ACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGA20080.4114324117766381No Hit
GAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTT19670.4030316503808004No Hit
GATTAACTCCATTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGG19350.3964749585596587No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA18920.3876644039249996No Hit
CCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAG18810.3854105411114822No Hit
GTGCTGGGAAGGACCCTAAGAAAACAGGAGGACCCATATATAGAAGAGTA18290.374755916902127No Hit
GTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCAC17090.35016832257284586No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA16560.33930880174408No Hit
ATGTGCAACTGATCCCCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGA16400.3360304558335092No Hit
ATATTGCACATTCTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACC15700.32168769247476187No Hit
GCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTCTT15540.318409346564191No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT15200.31144286150422806No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT15120.30980368854894264No Hit
TTCTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAA14960.30652534263837183No Hit
GTGTTGAACCTTGCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGA14880.30488616968308635No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC14830.303861686586033No Hit
TCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGACCCATATATAGAA14820.3036567899666223No Hit
TTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTC14730.30181272039192625No Hit
ATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATGA14660.3003784440560515No Hit
ATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATAT14630.2997637541978195No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA14090.28869933674964293No Hit
CAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATAC14000.2868552671749469No Hit
CTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAAACGATCAT13860.2839867145031974No Hit
GGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGC13820.2831671280255547No Hit
TAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAA13630.27927409225675187No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC13450.27558595310735967No Hit
ATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGA13260.2716929173385568No Hit
CTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTG13020.2667753984727006No Hit
CCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCACATTCTTT12430.2546864979274707No Hit
TATCATGATTTGGCATTCCAACCTGAATGATGCCACATATCAGAGAACAA12290.2518179452557212No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT11740.24054863118813402No Hit
AATGGAGACTGGTGGGGAGCGCCAGGATGCCACAGAAATCAGAGCATCTG11530.2362458021805098No Hit
GTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTTCCTGGG11410.2337870427475817No Hit
TACTTGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGA11350.23255766303111763No Hit
CTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCT11110.2276401441652614No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT11040.22620586782938668No Hit
TCTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCC11000.225386281351744No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT10690.21903448615001303No Hit
GATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAA10550.21616593347826354No Hit
GTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTGGGAAG10410.21329738080651406No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG10000.20489661941067633No Hit
GTCTTCAATCTCAGCGTTTCCTGGGTTTCGACTTTCTCTTACTTGATCCA9760.19997910054482013No Hit
ATCTCAGCGTTTCCTGGGTTTCGACTTTCTCTTACTTGATCCATCATTGC9560.19588116815660658No Hit
GAAATAAGGAGAGTTTGGCGCCAAGCAAACAATGGCGAAGATGCAACAGC9510.1948566850595532No Hit
CTCCAGAAATTTCGGTCATTGATTCCACGTTTGATCATTCTGATTAACTC9420.19301261548485713No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT9410.19280771886544645No Hit
CTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCAAACAATGG9320.19096364929075035No Hit
GTTTGAAATTTTCCTTTGAGGATATTGCACATTCTTTCATAAGCAACCCT9280.19014406281310767No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA9260.1897342695742863No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCC9250.18952937295487562No Hit
GAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTTCCTG9120.18686571690253684No Hit
ATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATACCT9120.18686571690253684No Hit
ATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCA8960.183587370991966No Hit
CTCAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGC8750.1792845419843418No Hit
GGCAGGATTTATGTGCAACTGATCCCCTCAGAATGAGTGCTGACCGTGCC8670.1776453690290564No Hit
CTTCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGG8220.16842502115557595No Hit
GAAGAAATAAGGAGAGTTTGGCGCCAAGCAAACAATGGCGAAGATGCAAC8210.16822012453616528No Hit
CAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGT8200.1680152279167546No Hit
GCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAAT8110.16617115834205853No Hit
CCATTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGAC7840.16063894961797026No Hit
GTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGA7590.15551653413270336No Hit
TTGCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTC7460.15285287808036457No Hit
ATTTTCACCCCTCCAGAAATTTCGGTCATTGATTCCACGTTTGATCATTC7380.15121370512507915No Hit
GGATATTGCACATTCTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCA7360.15080391188625777No Hit
CTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCACATTCTTTCAT7360.15080391188625777No Hit
ATTCTGATTAACTCCATTGCTATTGTTCCAACTCCTTTCACCGCAGCACC7280.14916473893097237No Hit
AAAGCAGGCAGGCAGGATTTATGTGCAACTGATCCCCTCAGAATGAGTGC7220.14793535921450832No Hit
CTTCAATCTCAGCGTTTCCTGGGTTTCGACTTTCTCTTACTTGATCCATC7180.1471157727368656No Hit
CCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTGGGAAGTGTTGAACC7070.1448619099233482No Hit
GTTTGGCGCCAAGCAAACAATGGCGAAGATGCAACAGCAGGTCTTACTCA7030.14404232344570547No Hit
AGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGC7000.14342763358747346No Hit
GGTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATA6830.13994439105749196No Hit
AATAAGGAGAGTTTGGCGCCAAGCAAACAATGGCGAAGATGCAACAGCAG6780.13891990796043857No Hit
ACATATCAGAGAACAAGAGCGCTTGTTCGCACCGGAATGGATCCCAGAAT6780.13891990796043857No Hit
TATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTGACA6660.13646114852751043No Hit
ATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTGAC6550.134207285713993No Hit
TCTGGGAAGTGTTGAACCTTGCATTAGAGAGCACATTCTGGGATCCATTC6480.13277300937811828No Hit
AACTTATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA6470.13256811275870758No Hit
CTCCATTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAG6460.1323632161392969No Hit
CATATGAACAAATGGAGACTGGTGGGGAGCGCCAGGATGCCACAGAAATC6440.13195342290047557No Hit
CTGATATGTGGCATCATTCAGGTTGGAATGCCAAATCATGATATGAGTAA6410.13133873304224353No Hit
GCTTAGTGACACCTCCTTGGCCCCATGGAACGTTATTTCTCTTTTGAGCT6300.12908487022872608No Hit
ACCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTCGGTCATTGAT6290.12887997360931544No Hit
CGTTTGATCATTCTGATTAACTCCATTGCTATTGTTCCAACTCCTTTCAC6290.12887997360931544No Hit
AATAAATACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAAAC6280.12867507698990474No Hit
GTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGAACGTTATTTCTCT6170.1264212141763873No Hit
GAACAAATGGAGACTGGTGGGGAGCGCCAGGATGCCACAGAAATCAGAGC6080.12457714460169121No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC6010.1231428682658165No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC5990.12273307502699513No Hit
CTGGTGGGGAGCGCCAGGATGCCACAGAAATCAGAGCATCTGTCGGAAGA5950.12191348854935241No Hit
GAAAATGAGGTCTTCAATCTCAGCGTTTCCTGGGTTTCGACTTTCTCTTA5930.12150369531053107No Hit
GAAATAAATACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAA5920.1212987986911204No Hit
TTGCACATTCTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACCCCT5890.12068410883288837No Hit
GAACAAGAGCGCTTGTTCGCACCGGAATGGATCCCAGAATGTGCTCTCTA5890.12068410883288837No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC5860.12006941897465633No Hit
TTTCCTGGGTTTCGACTTTCTCTTACTTGATCCATCATTGCCCTCTGGGC5850.11986452235524567No Hit
TAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAAAC5820.11924983249701362No Hit
GGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATACCTAGAAGAG5800.11884003925819228No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG5790.11863514263878161No Hit
CTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGC5770.11822534939996025No Hit
AAGCAACCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTCGGTCA5620.11515190010880011No Hit
TGATCATTCTGATTAACTCCATTGCTATTGTTCCAACTCCTTTCACCGCA5620.11515190010880011No Hit
ATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATA5570.11412741701174672No Hit
CAACTGATCCCCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCT5570.11412741701174672No Hit
AACAAATGGAGACTGGTGGGGAGCGCCAGGATGCCACAGAAATCAGAGCA5570.11412741701174672No Hit
GCTGGGAAGGACCCTAAGAAAACAGGAGGACCCATATATAGAAGAGTAGA5470.11207845081763995No Hit
CCTTTGAGGATATTGCACATTCTTTCATAAGCAACCCTTGTCCTTCGTCC5420.11105396772058658No Hit
GATTATGAGGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGT5400.11064417448176524No Hit
AGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAA5330.1092098981458905No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT5270.10798051842942644No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT5260.10777562181001575No Hit
AGCGAAAGCAGGCAGGCAGGATTTATGTGCAACTGATCCCCTCAGAATGA5260.10777562181001575No Hit
GAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAA5240.10736582857119441No Hit
ATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAAACGATCATATGA5200.10654624209355171No Hit
AGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATACCTAG5200.10654624209355171No Hit
TCCTTGGCCCCATGGAACGTTATTTCTCTTTTGAGCTTCTTGTATAGTTT5140.10531686237708765No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG5120.10490706913826629No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT5100.10449727589944495No Hit
GCACATTCTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACCCCTCC5080.10408748266062358No Hit
TTCCACGTTTGATCATTCTGATTAACTCCATTGCTATTGTTCCAACTCCT5060.10367768942180222No Hit
CTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATT5000.10244830970533816No Hit
ATCATGATATGAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTG4980.10203851646651682No Hit
GATATGTGGCATCATTCAGGTTGGAATGCCAAATCATGATATGAGTAAGA4970.10183361984710615No Hit
GAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAAACGATCATATGAAC4950.10142382660828478No Hit
CTCTTGTTCTCTGATATGTGGCATCATTCAGGTTGGAATGCCAAATCATG4920.10080913675005276No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC4920.10080913675005276No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT4910.10060424013064208No Hit
AACCTGAATGATGCCACATATCAGAGAACAAGAGCGCTTGTTCGCACCGG4890.10019444689182072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)