FastQCFastQC Report
Sat 5 Jun 2021
H2Y5VBGXJ_n01_S11_R2_D08_fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y5VBGXJ_n01_S11_R2_D08_fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794129
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT20870.2628036502885551No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA17160.21608579966227148No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA16440.20701926261350484No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC16390.20638964198511828No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT14270.17969372734152764No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC14250.179441879090173No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT14140.17805671370772255No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG13840.17427898993740312No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC13760.1732715969319846No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA13430.16911610078463324No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA12970.16332359100347676No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA12160.1531237368236143No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG12160.1531237368236143No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG11040.1390202347477551No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT11000.13851653824504584No Hit
AATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAAC10770.1356202833544676No Hit
ATATAGTGGTATTCCCTCTCCTTCATGACTTGGATCCTCAATGCTTAATT10740.13524251097743564No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC10570.13310180084092132No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC10120.12743521518544218No Hit
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC9720.12239825015834958No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC9320.11736128513125703No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC8880.11182062360145517No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAGTCATGG8780.11056138234468203No Hit
GTATAGAAAGCTTAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATAG8640.10879844458519963No Hit
CTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCGTTTAAGGAGA8640.10879844458519963No Hit
GTTTTTTACTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCAC8520.10728735507707185No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA8370.10539849319191215No Hit
GTTCTGGCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAG8290.10439110018649364No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG8140.10250223830133391No Hit
CAATAGGAGAGTGCCCAAAGTATGTCAGGAGTACAAAATTAAGGATGGTT8070.10162076942159272No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAGTGAGCAGGCA8020.10099114879320613No Hit
CGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCACAATATCAAGTG7960.10023560403914225No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)