FastQCFastQC Report
Sat 5 Jun 2021
H2Y5VBGXJ_n01_S11_R2_A08_fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y5VBGXJ_n01_S11_R2_A08_fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478259
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA42000.2841180063845375No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC36660.24799443128707488No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC33180.22445322504378462No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA29150.19719142585974447No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT26780.1811590526423313No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC26010.17595022252528142No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC25990.1758149282365269No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA25940.17547669251464054No Hit
CTCCTATTGTGACTGGGTGTACATTCTGGAAAGGAAGACTACTGTTTATA25480.17236492387328609No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG25440.172094335295777No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC25160.1702002152532134No Hit
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC24370.16485609084740901No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAGTCATGG23380.1581590235540592No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT22800.1542354891801775No Hit
ATATAGTGGTATTCCCTCTCCTTCATGACTTGGATCCTCAATGCTTAATT22430.1517325448382185No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA22170.14997371908440943No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG20450.13833841025151886No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC20320.1374589973746143No Hit
ATGTATAGGCTACCATGCGAACAACTCAACCGACACTGTTGACACAGTAC19620.13272369726820538No Hit
CAATAGGAGAGTGCCCAAAGTATGTCAGGAGTACAAAATTAAGGATGGTT19600.13258840297945082No Hit
CCACTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGA19460.13164134295816904No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG18670.12629721855236464No Hit
GTCAAAAATCACACTGAAGTTCGCTTTCAGTATGATGTTCTTATCCATTA18490.12507956995357375No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC18290.12372662706602836No Hit
GTATTAATGTATACCCCCTTCATTATATATTCTGTGGCTCTGCAATGAGA17960.12149427130157842No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA17470.11817956122709215No Hit
GAGCAAAAGCAGGGTGGCAAAGACATAATGGATCCCAACACTGTGTCAAG17150.11601485260701948No Hit
CAATATGTATAGGCTACCATGCGAACAACTCAACCGACACTGTTGACACA16910.11439132114196497No Hit
ATCCAGAACAGCTTAACAATAGAGAGAATGGTGCTCTCTGCTTTTGACGA16560.1120236710887605No Hit
ATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGACA16300.11026484533495146No Hit
CCCTAGGAGATCTGGAGCTGCTGGCGCTGCAGTCAAAGGAGTTGGAACAA16190.10952072674680147No Hit
GTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGCATTTCCTGGG16120.10904719673616058No Hit
ATATAAATCCTGGCCATGCAGACCTCAGTGCCAAGGAGGCACAGGATGTA15880.10742366527110608No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG15780.10674719382733337No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT15430.10437954377412889No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT15330.10370307233035618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)