FastQCFastQC Report
Sat 5 Jun 2021
H2Y5VBGXJ_n01_S11_R2_A06_fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y5VBGXJ_n01_S11_R2_A06_fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111710
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA34330.3088035548839176No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC31800.28604582130231804No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC26960.24250928749404072No Hit
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC26180.23549306923568197No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA24390.21939174784790996No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC23740.2135448992992777No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG22440.2018512022020131No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA22380.2013114931052163No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT22240.200052171879357No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC21470.1931259051371311No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAGTCATGG20050.18035278984627284No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT19760.17774419587842152No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA19700.1772044867816247No Hit
GAGCAAAAGCAGGGTGGCAAAGACATAATGGATCCCAACACTGTGTCAAG19670.17693463223322628No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC18500.1664103048456882No Hit
GTCAAAAATCACACTGAAGTTCGCTTTCAGTATGATGTTCTTATCCATTA18280.16443137149076648No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC17750.15966394113572785No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC17370.1562457835226813No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG16500.1484200016191273No Hit
ATATAGTGGTATTCCCTCTCCTTCATGACTTGGATCCTCAATGCTTAATT15930.14329276519955744No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG15330.13789567423158916No Hit
CTCCTATTGTGACTGGGTGTACATTCTGGAAAGGAAGACTACTGTTTATA15330.13789567423158916No Hit
ATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGACA13760.12377328619873888No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA13640.12269386800514523No Hit
CCCTAGGAGATCTGGAGCTGCTGGCGCTGCAGTCAAAGGAGTTGGAACAA13580.12215415890834841No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG13120.1180163891662394No Hit
ATGTATAGGCTACCATGCGAACAACTCAACCGACACTGTTGACACAGTAC13060.11747668006944258No Hit
CCACTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGA12980.11675706794038014No Hit
ATCCAGAACAGCTTAACAATAGAGAGAATGGTGCTCTCTGCTTTTGACGA12950.11648721339198173No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT12910.11612740732745051No Hit
GATTTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC12900.1160374558113177No Hit
GTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGCATTTCCTGGG12870.1157676012629193No Hit
ACAATAGAGAGAATGGTGCTCTCTGCTTTTGACGAGAGGAGGAATAAATA12740.11459823155319283No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT12740.11459823155319283No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG11890.10695235268190445No Hit
CAATATGTATAGGCTACCATGCGAACAACTCAACCGACACTGTTGACACA11720.10542317690764677No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG11400.10254472839139703No Hit
GACATAATGGATCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCT11390.10245477687526423No Hit
GTGTATGGACCTGCCGTAGCCAGTGGGTACGACTTCGAAAAAGAGGGATA11320.10182511626233459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)