FastQCFastQC Report
Wed 19 Apr 2023
H2WN3BGXT_n02_br320.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WN3BGXT_n02_br320.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTAGACTCGGTAAGAAATAGAGGGTGATGCAGTTGGAGTTCTCTTTA211.76470588235294No Hit
GGCTATGAGACAGGGTCTGGAGGCTAGGTTGGGGTCTTTCTTTTCCAGAA211.76470588235294No Hit
TATGTAGGGATGGTTCAATATATGGAAATCCATCAATGTAATCCAGTATG211.76470588235294No Hit
AAGTATATTAAGTTTATATCTGCCATTAATTTGCAGAATGGGTGACCCTT15.88235294117647No Hit
CCCTGGAGGGGTGGGGGGGGGAAGCCAAGTATGGGCTTTGGTTGCAGCTG15.88235294117647No Hit
TAGGCCTCACAACTGTCAACTAAATGCGAAAATAACATCATTTACCTAAG15.88235294117647No Hit
GTAGTCGGGAGACTGGAGGGAGCAGGCAGGTCGATGGTACTCAACGCCAT15.88235294117647No Hit
AGACTTGGCACAAAGTGTAACATGCTAGAATGTGTTCAATTCATGCCACT15.88235294117647No Hit
GGATATGAGACAGGGTCTGGAGGCTAGGTTGGGGTCAATCTTATCCAGAA15.88235294117647No Hit
CTACTCGGCCGGCTATGACGCGCTGAGGCCCTTCCCGGCCTCTTACGGGG15.88235294117647No Hit
AACTACTAGATTCTAGCACAATAACCCAGTTATCAAAAACTCAATTAATT15.88235294117647No Hit
AACTGAGCAGTCTGCTGAAAAAGTAGAGGATCTCCTGAGGGCAGGTTAAG15.88235294117647No Hit
TGACTGAGTTTCAAATGTCCTTATGCCAGCAACTAAAAAGACTCAAGGTA15.88235294117647No Hit
ATACTCAACCTTGAAAACAAGCAAAGTCACCAACAGAAGCTCCACGGGGC15.88235294117647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)