FastQCFastQC Report
Thu 7 Apr 2022
H2WMTDRX2_n02_cDNA_A2_220401.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WMTDRX2_n02_cDNA_A2_220401.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6529076
Sequences flagged as poor quality0
Sequence length104
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT239770.3672341997550649Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT196740.30132900888272707Clontech SMART CDS Primer II A (100% over 26bp)
ATGCGGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT151490.23202364316175828Clontech SMART CDS Primer II A (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)