FastQCFastQC Report
Thu 7 Apr 2022
H2WMTDRX2_n02_cDNA_A1_220401.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WMTDRX2_n02_cDNA_A1_220401.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6927690
Sequences flagged as poor quality0
Sequence length104
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT309000.4460361245956444Clontech SMART CDS Primer II A (96% over 26bp)
ATGCGGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT173710.2507473631181534Clontech SMART CDS Primer II A (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT118770.17144242886156857Clontech SMART CDS Primer II A (100% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)