FastQCFastQC Report
Thu 7 Apr 2022
H2WMTDRX2_n02_GDO_C_220401.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WMTDRX2_n02_GDO_C_220401.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202897976
Sequences flagged as poor quality0
Sequence length104
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAGCTCTGCGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG21033801.0366687935812626Illumina Single End PCR Primer 1 (100% over 35bp)
GCATAGCTCTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTA13801700.6802285696531541No Hit
GCATAGCTCAGACCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG9254270.4561045990917129Illumina Single End PCR Primer 1 (100% over 35bp)
GCAGAGCTCTGCGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG4768380.2350136799787495Illumina Single End PCR Primer 1 (100% over 35bp)
GCATAGCTCTTAAACAGCAAGCAGAGCGGCACTCCCCCCATATAAGAAAA4741590.23369331195299847No Hit
GCAGAGCTCTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTA4550200.22426049237672044No Hit
GCATAGCTCTTAAACCAGCAAGCAGAGCGGCACTCCCCCATATAAGAAAA4352210.214502386164759No Hit
GCATAGCTCTTAAACGATGCTATATGAGTGGCTACCCCCATATAAGAAAA3926920.19354160536327875No Hit
GCATAGCTCTTAAACAGCAAGCAGAGCGGCACTCCCCCCATATAAGAAAT3660030.18038770381819874No Hit
GCATAGCTCTTAAACAGCAAGCAGAGCGGCACTCCCCCCATATAAGAAAC3613300.17808457586585288No Hit
GCATAGCTCTTAAACCAGCAAGCAGAGCGGCACTCCCCCATATAAGAAAT3501820.17259018887403785No Hit
GCATAGCTCTTAAACGATGCTATATGAGTGGCTACCCCCATATAAGAAAT3430920.16909582183313648No Hit
GCATAGCTCTTAAACGATGCTATATGAGTGGCTACCCCCATATAAGAAAG3358310.16551717598208077No Hit
GGGGGGCTCTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTA3335670.1644013442499791No Hit
GCATAGCTCTTAAACAGCAAGCAGAGCGGCACTCCCCCCATATAAGAAAG3331320.16418695078555146No Hit
GCATAGCTCTTAAACCAGCAAGCAGAGCGGCACTCCCCCATATAAGAAAG3296990.16249496742145914No Hit
GCATAGCTCTTAAACGATGCTATATGAGTGGCTACCCCCATATAAGAAAC3283380.16182418694999698No Hit
GCATAGCTCTTAAACCAGCAAGCAGAGCGGCACTCCCCCATATAAGAAAC3259040.1606245692662799No Hit
GGGGAGCTCTGCGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG2868080.14135577182889197Illumina Single End PCR Primer 1 (100% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2846930.14031337601908853No Hit
GCATAGCTCAGTAAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG2801780.1380881197158911Illumina Single End PCR Primer 1 (100% over 35bp)
GCATAGCTCTTAAACCCAGCAAGCAGAGCGGCACTCCCCATATAAGAAAA2616680.12896530816059004No Hit
GCATAGCTCTTAAACCCAGCAAGCAGAGCGGCACTCCCCATATAAGAAAG2251140.11094935712912188No Hit
GCATAGCTCGTAAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG2181530.10751856883974041Illumina Single End PCR Primer 1 (100% over 35bp)
GCAGAGCTCAGACCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG2160340.10647420159578132Illumina Single End PCR Primer 1 (100% over 35bp)
GCATAGCTCTTAAACTGAGTTCGCGGGCCCTCTCCCCCCATATAAGAAAA2107780.1038837371152485No Hit
GCATAGCTCTTAAACCCAGCAAGCAGAGCGGCACTCCCCATATAAGAAAC2043240.10070282810509651No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)