FastQCFastQC Report
Thu 7 Apr 2022
H2WMTDRX2_n02_GDO_B_220401.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WMTDRX2_n02_GDO_B_220401.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119777888
Sequences flagged as poor quality0
Sequence length104
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAGCTCTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTA9165870.765238906199448No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5097460.4255760462231559No Hit
GCATAGCTCTTAAACAGCAAGCAGAGCGGCACTCCCCCCATATAAGAAAA3319930.2771738636767414No Hit
GCATAGCTCTTAAACCAGCAAGCAGAGCGGCACTCCCCCATATAAGAAAA2877960.2402747325115634No Hit
GCATAGCTCTTAAACGATGCTATATGAGTGGCTACCCCCATATAAGAAAA2792060.2331031250108534No Hit
GCATAGCTCTTGATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG2785020.23251537044967763Illumina Single End PCR Primer 1 (100% over 35bp)
GCATAGCTCTTAAACAGCAAGCAGAGCGGCACTCCCCCCATATAAGAAAT2672320.22310628819903722No Hit
GCAGAGCTCTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTA2635230.22000972333057No Hit
GCATAGCTCTTAAACAGCAAGCAGAGCGGCACTCCCCCCATATAAGAAAG2524770.2107876538948491No Hit
GCATAGCTCTTAAACAGCAAGCAGAGCGGCACTCCCCCCATATAAGAAAC2519450.21034349845941513No Hit
GCATAGCTCTTAAACGATGCTATATGAGTGGCTACCCCCATATAAGAAAG2417880.20186363613290625No Hit
GCATAGCTCTTAAACGATGCTATATGAGTGGCTACCCCCATATAAGAAAT2369860.19785454891306817No Hit
GCATAGCTCTTAAACGATGCTATATGAGTGGCTACCCCCATATAAGAAAC2342540.19557366047395996No Hit
GCATAGCTCTTAAACCAGCAAGCAGAGCGGCACTCCCCCATATAAGAAAT2320230.19371104623250662No Hit
GCATAGCTCTTAAACCAGCAAGCAGAGCGGCACTCCCCCATATAAGAAAG2197940.1835013153679918No Hit
GCATAGCTCTTAAACCAGCAAGCAGAGCGGCACTCCCCCATATAAGAAAC2190280.18286179833125793No Hit
GCATAGCTCTTAAACCCAGCAAGCAGAGCGGCACTCCCCATATAAGAAAA2059050.17190568596434092No Hit
GCATAGCTCTTAAACCCAGCAAGCAGAGCGGCACTCCCCATATAAGAAAG1696350.14162463776285653No Hit
GCATAGCTCTTAAACCCAGCAAGCAGAGCGGCACTCCCCATATAAGAAAC1687820.14091248628461372No Hit
GCATAGCTCTTAAACCCAGCAAGCAGAGCGGCACTCCCCATATAAGAAAT1622120.13542733363273196No Hit
GCATAGCTCTTAAACAGCAAAGCCGGAGCCGGAGCCCCCATATAAGAAAA1478490.12343597175465308No Hit
GCATAGCTCTTAAACCTGCCGGGTGGTACCGAGCCCCCCATATAAGAAAA1478210.12341259515278814No Hit
GCATAGCTCTTAAACTGAGTTCGCGGGCCCTCTCCCCCCATATAAGAAAA1458560.12177205862905179No Hit
GCATAGCTCTTAAACTGAGTTCGCGGGCCCTCTCCCCCCATATAAGAAAC1304580.10891659736060799No Hit
GCATAGCTCTTAAACTGAGTTCGCGGGCCCTCTCCCCCCATATAAGAAAG1263200.10546186955642431No Hit
GCATAGCTCTTAAACTGAGTTCGCGGGCCCTCTCCCCCCATATAAGAAAT1258080.10503441169375102No Hit
GCATAGCTCTTAAACAGCAAAGCCGGAGCCGGAGCCCCCATATAAGAAAG1254650.10474804832090542No Hit
GCATAGCTCTTAAACCTGCCGGGTGGTACCGAGCCCCCCATATAAGAAAG1248620.10424461650217108No Hit
GCATAGCTCTTAAACCTGCCGGGTGGTACCGAGCCCCCCATATAAGAAAC1214410.1013884966814576No Hit
GCATAGCTCTTAAACCTGCCGGGTGGTACCGAGCCCCCCATATAAGAAAT1211700.10116224457055044No Hit
GCATAGCTCTTAAACAGCAAAGCCGGAGCCGGAGCCCCCATATAAGAAAT1209650.10099109444975353No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)