FastQCFastQC Report
Thu 7 Apr 2022
H2WMTDRX2_n02_GDO_A_220401.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WMTDRX2_n02_GDO_A_220401.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251449856
Sequences flagged as poor quality0
Sequence length104
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAGCTCTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTA12021450.47808538017218033No Hit
GCATAGCTCTTAAACAGCAAGCAGAGCGGCACTCCCCCCATATAAGAAAA7989650.3177432720422795No Hit
GCATAGCTCTTAAACAGCAAGCAGAGCGGCACTCCCCCCATATAAGAAAT6453510.25665196642626037No Hit
GCATAGCTCTTAAACAGCAAGCAGAGCGGCACTCCCCCCATATAAGAAAC6207300.24686035214909807No Hit
GCATAGCTCTTAAACAGCAAGCAGAGCGGCACTCCCCCCATATAAGAAAG6148270.2445127667919603No Hit
GCATAGCTCTTAAACGATGCTATATGAGTGGCTACCCCCATATAAGAAAA5913190.23516378549844943No Hit
GCATAGCTCTTAAACCAGCAAGCAGAGCGGCACTCCCCCATATAAGAAAA5873840.23359886115822634No Hit
GCATAGCTCTTAAACGATGCTATATGAGTGGCTACCCCCATATAAGAAAC4825310.19189949347197083No Hit
GCATAGCTCTTAAACGATGCTATATGAGTGGCTACCCCCATATAAGAAAT4732420.18820531756438946No Hit
GCATAGCTCTTAAACGATGCTATATGAGTGGCTACCCCCATATAAGAAAG4634410.18430752253045632No Hit
GCATAGCTCTTCGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG4613800.18348787600816918Illumina Single End PCR Primer 1 (100% over 35bp)
GCATAGCTCTTAAACCAGCAAGCAGAGCGGCACTCCCCCATATAAGAAAT4613120.1834608328429506No Hit
GCATAGCTCTTAAACCAGCAAGCAGAGCGGCACTCCCCCATATAAGAAAC4389770.17457834614946052No Hit
GCATAGCTCTTAAACCAGCAAGCAGAGCGGCACTCCCCCATATAAGAAAG4302360.17110210633805256No Hit
GCATAGCTCTTAAACCTGCCGGGTGGTACCGAGCCCCCCATATAAGAAAA4270140.1698207375390185No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4242970.16874020401109316No Hit
GCATAGCTCTTAAACCTGCCGGGTGGTACCGAGCCCCCCATATAAGAAAG3714240.14771294997281686No Hit
GCATAGCTCTTAAACCCAGCAAGCAGAGCGGCACTCCCCATATAAGAAAA3709030.14750575160400967No Hit
GCAGAGCTCTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTA3478600.13834169783736125No Hit
GCATAGCTCTTAAACAGCAAAGCCGGAGCCGGAGCCCCCATATAAGAAAA3419300.1359833747528573No Hit
GCATAGCTCTTAAACCTGCCGGGTGGTACCGAGCCCCCCATATAAGAAAC3312910.1317523124769656No Hit
GCATAGCTCTTAAACCTGCCGGGTGGTACCGAGCCCCCCATATAAGAAAT3265820.12987957328557787No Hit
GCATAGCTCTTAAACCCAGCAAGCAGAGCGGCACTCCCCATATAAGAAAC3074510.122271296906211No Hit
GCATAGCTCTTAAACCCAGCAAGCAGAGCGGCACTCCCCATATAAGAAAG2968100.11803943924310697No Hit
GCATAGCTCTTAAACTGAGTTCGCGGGCCCTCTCCCCCCATATAAGAAAA2902620.11543534150999872No Hit
GCATAGCTCTTAAACAGCAAAGCCGGAGCCGGAGCCCCCATATAAGAAAT2867140.11402432459535788No Hit
GCATAGCTCTAAGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG2795040.11115695369497448Illumina Single End PCR Primer 1 (100% over 35bp)
GCATAGCTCTTAAACCCAGCAAGCAGAGCGGCACTCCCCATATAAGAAAT2694520.10715933756589623No Hit
GCATAGCTCTTAAACAGCAAAGCCGGAGCCGGAGCCCCCATATAAGAAAG2646800.1052615436773207No Hit
GCATAGCTCTTAAACTGAGTTCGCGGGCCCTCTCCCCCCATATAAGAAAT2613730.10394637092176343No Hit
GCATAGCTCTTAAACTGAGTTCGCGGGCCCTCTCCCCCCATATAAGAAAC2588890.10295850000407239No Hit
GCATAGCTCTTAAACACCCCTCCCCACATCAAGCCCGAATGATATTTCCT2538220.10094338650168087No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)