FastQCFastQC Report
Thu 7 Apr 2022
H2WMTDRX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WMTDRX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42015934
Sequences flagged as poor quality0
Sequence length26
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAA3331520.7929182295459622No Hit
ATCTCGTATGCCGTCTTCTGCTTGAA2147950.5112227185048416Illumina Single End Adapter 1 (95% over 24bp)
GATCTCGTATGCCGTCTTCTGCTTGA1326830.3157920992545352Illumina Single End Adapter 1 (96% over 25bp)
AGATCTCGTATGCCGTCTTCTGCTTG742990.17683529301050407Illumina Single End Adapter 1 (96% over 26bp)
GTATCTCGTATGCCGTCTTCTGCTTG676980.16112458668656515Illumina PCR Primer Index 2 (100% over 26bp)
TGCGCAGAGCTATGCTGGAATTCTCG656520.1562550055414691No Hit
TTATCTCGTATGCCGTCTTCTGCTTG448090.10664763515670031Illumina PCR Primer Index 2 (96% over 26bp)
TATCTCGTATGCCGTCTTCTGCTTGA428450.1019732180653178Illumina Paired End PCR Primer 2 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)