FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_down_unknown.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_down_unknown.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17474410
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG11600.015.7528538
ACCTAGG38100.014.65982810
AGTTACG24600.013.0361726
CGCGTAT24550.012.92688751
ATACCTA47600.012.820588
TAAGTCG22100.012.79268559
TACCTAG46500.012.3823669
GTCCCTA26200.012.3699498
TAGGTAC47250.012.11302513
CGCGAAT23950.012.0984961
CTATAAG34100.011.83797546
CGCTATG25800.011.63820629
CTACACG25950.010.89495212
GTCGCTA28450.010.79439827
CCTAGGT52000.010.7413611
TACGGGG20750.010.63413059
GTCCTAT38500.010.57907243
TATAAGC39750.010.50166347
TAGGACC33350.010.44712649
CGCGATA20200.010.4168431
Produced by FastQC (version 0.11.2)