FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_25_50_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_25_50_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15376098
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCCTA34000.012.5739868
CGCGAAT17500.012.4186471
TCCCTAC32050.012.2632519
CTACACG27150.012.19090312
CGCGTAT11400.011.8013461
CCCTACA34700.011.12800610
ACCTAGG21850.010.57187910
CCGGGAT69050.010.3413791
GGAAAAT89750.010.185521
CGGAAAT23450.010.1502811
TACACGA32100.09.88143613
CGGGGAT50650.09.8755281
CGGGCAT33200.09.55921
CGACAAT50700.09.321471
GGGAAAT45700.08.982611
CCGGAAT30900.08.9310661
CGGGACT17550.08.845191
CGGGTAT12150.08.801511
GCCGAAT25900.08.6573581
ACACGAC38200.08.57424914
Produced by FastQC (version 0.11.2)