FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_25_50_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_25_50_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10767792
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG5950.017.3843948
ACCTAGG22450.016.12605710
ATACCTA26200.014.7391358
TACCTAG25550.014.4393659
CTACACG16750.013.380115512
TAGGTAC27250.013.03268913
TACGGGG10700.012.8893639
AGTTACG12850.012.6123616
CGCTATG17550.012.58594629
GTCGCTA17200.012.23706927
CCCTACA23150.012.21288110
CCTAGGT30050.012.16255311
TCCCTAC21550.012.1596649
CGCGAAT12750.011.9042351
CGGGCAT18000.011.6900481
CGGGACT13900.011.167521
GTACCCA32150.011.15382416
GTGGATA31300.011.1269644
GTCCCTA16350.010.7549318
GCGGAAT18350.010.7151381
Produced by FastQC (version 0.11.2)