FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_25_50_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_25_50_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11390924
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG7900.013.9665158
CGCGTAT17600.013.7218211
ACCTAGG29850.013.63021810
TACCTAG31700.013.3786119
TAAGTCG16050.013.3193139
CGCGAAT15100.012.7949171
CGCTATG20300.012.7507229
GTCGCTA20000.012.42157327
ATACCTA34250.012.3825398
TAGGTAC33800.012.24141413
CGGGACT14800.011.65560151
GTCCCTA18700.011.4318168
CCTAGGT35950.011.41360311
GCGTATT20650.010.8554552
GTACCCA39550.010.72337216
GCGGAAT16900.010.6155621
CTATGCG16300.010.58354
CGCGATA12750.010.553121
GTGGATA39600.010.45472051
CTAGGTA41800.010.3936712
Produced by FastQC (version 0.11.2)