FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_25_25_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_25_25_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13004608
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG23350.013.1420212
TCCCTAC28550.012.6803779
ACCTAGG20750.012.62833710
CCCTACA31750.012.48834110
TTCCCTA31800.011.7097428
TACCTAG27100.011.5776689
ATACCTA26800.011.0640558
CGCGAAT18250.010.9628771
CTATAAG19400.010.84928646
GCGGAAT18300.010.7444261
CGGAAAT23550.010.6927991
CCGGGAT57550.010.54937651
GCCGAAT20100.010.4687121
TACACGA30800.010.18711913
CGTAATC13300.010.12422638
CGGGCAT25400.010.04981
ACACTCT34350.09.8414431
TTACGGG7759.094947E-129.78749758
CCTAGGT26800.09.77772411
CTCGAAT21900.09.7657811
Produced by FastQC (version 0.11.2)