FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_25_25_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_25_25_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13007190
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG29500.015.30992610
TTACGGG9750.013.4370678
CGGGACT16650.013.2591811
CGCGAAT17150.013.07375051
TAGGTAC32950.012.97503313
TACCTAG36150.012.9704329
ATACCTA34600.012.5550948
AGTTACG18900.012.0410526
CGGAATA16800.011.9118768
CTATAAG21450.011.42068846
GTGGATA37500.011.4017374
TAAGTCG16850.011.0488659
CGCTATG18800.011.01539229
CCTAGGT40550.010.88319211
CGCGTAT20000.010.8657941
GGCGAAT14750.010.7576331
CCCTACA25700.010.73200110
CTAGGTA45100.010.32046912
ATGCGTG24450.010.2949986
GTCGCTA19550.010.23683727
Produced by FastQC (version 0.11.2)