FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_25_25_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_25_25_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14826968
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG42350.020.4347210
ATACCTA47000.019.1466358
TACCTAG48650.017.9302379
TAGGTAC47600.017.6741513
GTGGATA50900.017.0723234
GGATACC58200.015.28634556
GTACCCA56650.015.2160416
CCTAGGT58000.015.0399111
CTAGGTA63000.013.84626712
CTATAAG26450.013.69695546
TTACGGG13150.013.6341298
GATACCT64700.013.5889897
CGCGAAT22050.013.4554731
TAAGTCG22300.013.4512539
CGGGACT19800.013.4163581
CTACACG22900.013.40007612
CGGAATA17500.013.01178868
GCGTAGC70950.012.31267936
GTCGCTA25800.012.037003527
CGCGTAT26750.011.9941171
Produced by FastQC (version 0.11.2)