FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_25_125_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_25_125_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12201007
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG25800.017.90802610
TAGGTAC30150.016.01050813
ATACCTA29950.015.8871098
TACCTAG32000.015.5158349
CCTAGGT34300.013.97296611
TTACGGG7750.013.7918518
CTACACG18600.013.71781412
CGCGAAT16450.013.4214971
CCCTACA24700.013.12180210
TCCCTAC22650.012.6349199
CTAGGTA38000.012.52165112
GTGGATA38100.012.489764
CGCGTAT16450.012.3729431
CGCGATA13550.012.2204961
GTACCCA40200.011.75085716
GGATACC43000.011.7081896
GCGAATC25150.011.5179472
CTATGCG18950.011.47031154
CGGGACT16500.010.8719221
TAAGTCG14100.010.7596029
Produced by FastQC (version 0.11.2)